1,013 research outputs found
What is the research evidence for antibiotic resistance exposure and transmission to humans from the environment? A systematic map protocol
This is the final version. Available from the publisher via the DOI in this record.Background: Antimicrobial resistance (AMR) is a public health crisis that is predicted to cause 10 million deaths per
year by 2050. The environment has been implicated as a reservoir of AMR and is suggested to play a role in the dissemination of antibiotic resistance genes (ARGs). Currently, most research has focused on measuring concentrations
of antibiotics and characterising the abundance and diversity of ARGs and antibiotic resistant bacteria (ARB) in the
environment. To date, there has been limited empirical research on whether humans are exposed to this, and whether
exposure can lead to measureable impacts on human health. Therefore, the objective of this work is to produce two
linked systematic maps to investigate previous research on exposure and transmission of AMR to humans from the
environment. The frst map will investigate the available research relating to exposure and transmission of ARB/ARGs
from the environment to humans on a global scale and the second will investigate the prevalence of ARB/ARGs in
various environments in the UK. These two maps will be useful for policy makers and research funders to identify
where there are signifcant gluts and gaps in the current research, and where more primary and synthesis research
needs to be undertaken.
Methods: Separate search strategies will be developed for the two maps. Searches will be run in 13 databases, and
grey literature will be sought from key websites and engagement with experts. Hits will be managed in EndNote and
screened in two stages (title/abstract then full text) against predefned inclusion criteria. A minimum of 10% will be
double screened with ongoing consistency checking. All included studies will have data extracted into a bespoke
form designed and piloted for each map. Data to be extracted will include bibliographic details, study design, location, exposure source, exposure route, health outcome (Map 1); and prevalence/percentage of ARB/ARG (Map 2).
No validity appraisal will be undertaken. Results will be tabulated and presented narratively, together with graphics
showing the types and areas of research that has been undertaken and heatmaps for key exposure-health outcomes
(Map 1) and exposure-prevalence (Map 2).Natural Environment Research Council (NERC
A coliform-targeted metagenomic method facilitating human exposure estimates to Escherichia coli-borne antibiotic resistance genes.
This is the author accepted manuscript. The final version is available from Oxford University Press via the DOI in this record.Background: Antimicrobial resistance and the spread of antibiotic resistance genes (ARGs) pose a threat to human health. Community-acquired infections resistant to treatment with first-line antibiotics are increasing, and there are few studies investigating environmental exposures and transmission. Aim: Our objective is to develop a novel targeted metagenomic method to quantify the abundance and diversity of ARGs in a faecal indicator bacterium, and to estimate human exposure to resistant bacteria in a natural environment. Approach: Sequence data from Escherichia coli metagenomes from 13 bathing waters in England were analysed using the ARGs Online Analysis Pipeline to estimate the abundance and diversity of resistance determinants borne by this indicator bacterium. These data were averaged over the 13 sites and used along with data on the levels of E. coli in English bathing waters in 2016 and estimates of the volume of water that water users typically ingest in an average session of their chosen activityto quantify the numbers of ARGs that water users ingest. Findings: Escherichia coli in coastal bathing waters were found to harbour on average 1.24 ARGs per cell. Approximately 2.5 million water sports sessions occurred in England in 2016 that resulted in water users ingesting at least 100 E. coli-borne ARGs.This work was supported by the University of Exeter and the Chinese University of Hong Kong
A cross-sectional study on the prevalence of illness in coastal bathers compared to non-bathers in England and Wales: Findings from the Beach User Health Survey
This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this record.The risks of illness associated with bathing in UK coastal waters have not been quantified since the early 1990s. Efforts have been made since then to improve the quality of bathing waters. The aim of this study was to quantify the prevalence of symptoms of illness associated with sea bathing in bathers in England and Wales. A cross-sectional study was conducted between June 2014 and April 2015. An online survey collected information from sea bathers and non-bathers on their visits to beaches in England and Wales along with the occurrence of symptoms of illness. 2631 people (1693 bathers, 938 non-bathers) responded to the survey. Compared to non-bathers, bathers were more likely to report skin ailments (adjusted prevalence odds ratio (AOR) = 2.64, 95% confidence interval (CI) 1.23 to 5.65, p = 0.01), ear ailments (AOR = 3.77, 95% CI 1.84 to 7.73, p < 0.001), and any symptoms of illness (AOR = 3.73, 95% CI 2.63 to 5.29, p < 0.001). There was weak evidence of an increase in the odds of gastrointestinal illness (AOR = 1.59, 95% CI 0.96 to 2.65, p = 0.07), respiratory ailments (AOR = 2.44, 95% CI 0.92 to 6.48, p = 0.07) and eye ailments (AOR = 2.12, 95% CI 0.83 to 5.39, p = 0.11). While the study design does not allow inference of causality, we do observe an association between sea bathing in England and Wales and reported symptoms of ill health. This suggests that despite higher rates of compliance with water quality criteria among bathing waters nowadays, the odds of illness for bathers relative to non-bathers is similar in magnitude to estimates made in the 1990s.Natural Environment Research Council (NERC)European Regional Development FundNational Institute for Health Researc
Natural recreational waters and the risk that exposure to antibiotic resistant bacteria poses to human health
This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this recordAntimicrobial resistance (AMR) is widely recognised as a considerable threat to human health, wellbeing and prosperity. Many clinically important antibiotic resistance genes are understood to have originated in the natural environment. However, the complex interactions between humans, animals and the environment makes the health implications of environmental AMR difficult to quantify. This narrative review focuses on the current state of knowledge regarding antibiotic resistant bacteria (ARB) in natural bathing waters and implications for human health. It considers the latest research focusing on the transmission of ARB from bathing waters to humans. The limitations of existing evidence are discussed, as well as research priorities. The authors are of the opinion that future studies should include faecally contaminated bathing waters and people exposed to these environments to accurately parameterise environment-to-human transmission.Natural Environment Research CouncilMedical Research CouncilNatural Environment Research CouncilNatural Environment Research CouncilEnvironmental Protection Agenc
Seasonal variation in collective mood via Twitter content and medical purchases
The analysis of sentiment contained in vast amounts of Twitter messages has reliably shown seasonal patterns of variation in multiple studies, a finding that can have great importance in the understanding of seasonal affective disorders, particularly if related with known seasonal variations in certain hormones. An important question, however, is that of directly linking the signals coming from Twitter with other sources of evidence about average mood changes. Specifically we compare Twitter signals relative to anxiety, sadness, anger, and fatigue with purchase of items related to anxiety, stress and fatigue at a major UK Health and Beauty retailer. Results show that all of these signals are highly correlated and strongly seasonal, being under-expressed in the summer and over-expressed in the other seasons, with interesting differences and similarities across them. Anxiety signals, extracted from both Twitter and from Health product purchases, peak in spring and autumn, and correlate also with the purchase of stress remedies, while Twitter sadness has a peak in the Winter, along with Twitter anger and remedies for fatigue. Surprisingly, purchase of remedies for fatigue do not match the Twitter fatigue, suggesting that perhaps the names we give to these indicators are only approximate indications of what they actually measure. This study contributes both to the clarification of the mood signals contained in social media, and more generally to our understanding of seasonal cycles in collective mood
Emerging technologies in physics education
Three emerging technologies in physics education are evaluated from the
interdisciplinary perspective of cognitive science and physics education
research. The technologies - Physlet Physics, the Andes Intelligent Tutoring
System (ITS), and Microcomputer-Based Laboratory (MBL) Tools - are assessed
particularly in terms of their potential at promoting conceptual change,
developing expert-like problem-solving skills, and achieving the goals of the
traditional physics laboratory. Pedagogical methods to maximize the potential
of each educational technology are suggested.Comment: Accepted for publication in the Journal of Science Education and
Technology; 20 page
Existing evidence on antibiotic resistance exposure and transmission to humans from the environment: a systematic map
This is the final version. Available on open access from BMC via the DOI in this recordAvailability of data and materials;
All data is provided as additional files.Background
Antimicrobial resistance (AMR) is predicted to become the leading cause of death by 2050 with antibiotic resistance being an important component. Anthropogenic pollution introduces antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) to the natural environment. Currently, there is limited empirical evidence demonstrating whether humans are exposed to environmental AMR and whether this exposure can result in measurable human health outcomes. In recent years there has been increasing interest in the role of the environment and disparate evidence on transmission of AMR to humans has been generated but there has been no systematic attempt to summarise this. We aim to create two systematic maps that will collate the evidence for (1) the transmission of antibiotic resistance from the natural environment to humans on a global scale and (2) the state of antibiotic resistance in the environment in the United Kingdom.
Methods
Search strategies were developed for each map. Searches were undertaken in 13 bibliographic databases. Key websites were searched and experts consulted for grey literature. Search results were managed using EndNote X8. Titles and abstracts were screened, followed by the full texts. Articles were double screened at a minimum of 10% at both stages with consistency checking and discussion when disagreements arose. Data extraction occurred in Excel with bespoke forms designed. Data extracted from each selected study included: bibliographic information; study site location; exposure source; exposure route; human health outcome (Map 1); prevalence/percentage/abundance of ARB/antibiotic resistance elements (Map 2) and study design. EviAtlas was used to visualise outputs.
Results
For Map 1, 40 articles were included, from 11,016 unique articles identified in searches, which investigated transmission of AMR from the environment to humans. Results from Map 1 showed that consumption/ingestion was the most studied transmission route. Exposure (n = 17), infection (n = 16) and colonisation (n = 11) being studied as an outcome a similar number of times, with mortality studied infrequently (n = 2). In addition, E. coli was the most highly studied bacterium (n = 16). For Map 2, we included 62 studies quantifying ARB or resistance elements in the environment in the UK, from 6874 unique articles were identified in the searches. The most highly researched species was mixed communities (n = 32). The most common methodology employed in this research question was phenotypic testing (n = 37). The most commonly reported outcome was the characterisation of ARBs (n = 40), followed by characterisation of ARGs (n = 35). Other genetic elements, such as screening for intI1 (n = 15) (which encodes a Class 1 integron which is used as a proxy for environmental ARGs) and point mutations (n = 1) were less frequently reported. Both maps showed that research was focused towards aquatic environments.
Conclusions
Both maps can be used by policy makers to show the global (Map 1) and UK (Map 2) research landscapes and provide an overview of the state of AMR in the environment and human health impacts of interacting with the environment. We have also identified (1) clusters of research which may be used to perform meta-analyses and (2) gaps in the evidence base where future primary research should focus.Natural Environment Research Council (NERC
Two distinct sequences of blue straggler stars in the globular cluster M30
Stars in globular clusters are generally believed to have all formed at the
same time, early in the Galaxy's history. 'Blue stragglers' are stars massive
enough that they should have evolved into white dwarfs long ago. Two possible
mechanisms have been proposed for their formation: mass transfer between binary
companions and stellar mergers resulting from direct collisions between two
stars. Recently, the binary explanation was claimed to be dominant. Here we
report that there are two distinct parallel sequences of blue stragglers in
M30. This globular cluster is thought to have undergone 'core collapse', during
which both the collision rate and the mass transfer activity in binary systems
would have been enhanced. We suggest that the two observed sequences arise from
the cluster core collapse, with the bluer population arising from direct
stellar collisions and the redder one arising from the evolution of close
binaries that are probably still experiencing an active phase of mass transfer.Comment: Published on the 24th December 2009 issue of Natur
Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects
The medical significance of circulating endothelial or hematopoietic progenitors is becoming increasing recognized. While therapeutic augmentation of circulating progenitor cells using G-CSF has resulted in promising preclinical and early clinical data for several degenerative conditions, this approach is limited by cost and inability to perform chronic administration. Stem-Kine is a food supplement that was previously reported to augment circulating EPC in a pilot study. Here we report a trial in 18 healthy volunteers administered Stem-Kine twice daily for a 2 week period. Significant increases in circulating CD133 and CD34 cells were observed at days 1, 2, 7, and 14 subsequent to initiation of administration, which correlated with increased hematopoietic progenitors as detected by the HALO assay. Augmentation of EPC numbers in circulation was detected by KDR-1/CD34 staining and colony forming assays. These data suggest Stem-Kine supplementation may be useful as a stimulator of reparative processes associated with mobilization of hematopoietic and endothelial progenitors
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